| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas
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| Autores: |
Boullot, Floriane
Castrec, Justine
Bidault, Adeline
Dantas, Natanael
Payton, Laura
Perrigault, Mickael
Tran, Damien
Amzil, Zouher
Boudry, Pierre
Soudant, Philippe
Hegaret, Helene
Fabioux, Caroline
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| Data: |
2017-01
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| Ano: |
2017
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| Palavras-chave: |
Crassostrea gigas
Sodium channel
Alternative splicing
Alexandrium minutum
Paralytic shellfish toxins
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| Resumo: |
Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00368/47877/47892.pdf
DOI:10.3390/md15010021
https://archimer.ifremer.fr/doc/00368/47877/
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| Editor: |
Mdpi Ag
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| Formato: |
application/pdf
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| Fonte: |
Marine Drugs (1660-3397) (Mdpi Ag), 2017-01 , Vol. 15 , N. 1 , P. 21 (1-23)
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| Direitos: |
2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
info:eu-repo/semantics/openAccess
restricted use
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